Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/01/29

<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment:&nbsp;</strong>Optimisation of HTT-HAP40 purification using heparin affinity chromatography</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/01/29</p> <p><strong>Rationale:&nbsp;</strong>The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin.&nbsp;To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems were conducted using heparin and FLAG affinity chromatography which showed the sample bound heparin resin &ndash; see&nbsp;<a href="https://zenodo.org/record/2553669">https://zenodo.org/record/2553669</a>. Now this need to be scaled up and tested more stringently.&nbsp;</p>