Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation 2019/04/15

<p><strong>Project:&nbsp;</strong>High resolution structural analysis of purified HTT samples</p> <p><strong>Experiment:&nbsp;</strong>Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/04/15</p> <p><strong>Rationale:&nbsp;</strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM.&nbsp;</p>