Large-scale stringent purification of HTT-HAP40 using heparin affinity chromatography 2019/05/12

<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment:&nbsp;</strong>Large-scale stringent purification of HTT-HAP40 using heparin affinity chromatography</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/05/12</p> <p><strong>Rationale:&nbsp;</strong>Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In small-scale experiment, I also incorporated helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment included 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step and yielded a highly pure HTT-HAP40 sample:&nbsp;<a href="https://zenodo.org/record/2628064">https://zenodo.org/record/2628064</a>.&nbsp;</p>