Interaction of hypertensive rat serum immunoglobulin with recombinant human proteins

<p><strong>Microsoft Excel file: &ldquo;HuProt supplemental data.xlsx&rdquo;</strong></p> <p><em>Description of supplementary material : HuProt array v3.1 IgM and IgG signals from rat serum pools.</em></p> <p><strong>Sheet 1</strong>: &ldquo;Array Z scores&rdquo; Z scores for all array features for all sample pools used in the study detected with anti-IgM and anti-IgG reporter antibodies</p> <p><strong>Sheet 2</strong>: &ldquo;IgM Z scores top 35&rdquo; Z scores for the 35 array features that had highest scores detected with anti-IgM reporter in 28 week old salt loaded SHR-A3 and the corresponding scores for 16 week old SHR-A3 and 28 week old SHR-A3(<em>IgH</em>-B2) congenics.</p> <p><strong>Sheet 3</strong>: &ldquo;IgM Z scores higher at 28 wks&rdquo; Refinement of Sheet 2 to eliminate array features in which IgM Z scores were greater in 16 week old SHR-A3 prior to salt loading compared with 28 week old salt-loaded SHR-A3.</p> <p><strong>Sheet 4</strong>: &ldquo;IgM Z scores congenics down&rdquo; Refinement of Sheet 3 to include only array features in which IgM Z scores were reduced in 28 week old salt-loaded SHR-A3(<em>IgH</em>-B2) compared with 28 week old salt-loaded SHR-A3.</p> <p><strong>Sheet 5</strong>: &ldquo;IgG Z scores top 35&rdquo; Z scores for the 35 array features that had highest scores detected with anti-IgG reporter in 28 week old salt loaded SHR-A3 and the corresponding scores for 16 week old SHR-A3 and 28 week old SHR-A3(<em>IgH</em>-B2) congenics.</p> <p><strong>Sheet 6</strong>: &ldquo;IgG Z scores higher at 28 wks&rdquo; Refinement of Sheet 2 to eliminate array features in which IgG Z scores were greater in 16 week old SHR-A3 prior to salt loading compared with 28 week old salt-loaded SHR-A3.</p> <p><strong>Sheet 7</strong>: &ldquo;IgG Z scores congenics down&rdquo; Refinement of Sheet 3 to include only array features in which IgG Z scores were reduced in 28 week old salt-loaded SHR-A3(<em>IgH</em>-B2) compared with 28 week old salt-loaded SHR-A3.</p> <p><strong>Sheet 8:&nbsp;</strong>&ldquo;Table 1 IgM IgG concordance&rdquo; This data is presented as Table 1 in the manuscript and identifies those array features that are common to both Sheet 4 and Sheet 7, representing array features for which both IgM and IgG reactivity were reduced in 28 week old salt-loaded SHR-A3(<em>IgH</em>-B2) compared with 28 week old salt-loaded SHR-A3.</p>