zenodoopen
Recombinant PNGaseF production and deglycosylation assays
<p>Recombinant PNGaseF production and deglycosylation assays. Open Lab notebook.</p> <p>1.5 L of BL21 E.coli cells harbouring the pOPH6:EmPNGaseF vector encoding a His-tagged construct of Elizabethkingia miricola PNGase F (EmPNGaseF, Uniprot P21163, PNGF_ELIMR) were used to express the protein. EmPNGAse F was purified from the E.coli lysate by immobilised metal affinity and size exclusion chromatography and its activity assayed against 5 distinct glycoproteins.</p>
ShareScore
40/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 8
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 8
- Engagement
- 4