ImageInLife20170922IP01_Sipka_Macrophage Recruitment to the Wound

<p>A tail of 3dpf transgenic zebrafish larvae (<em>tg(mpeg1:Gal4/UAS:Kaede</em>)) was imaged from 1h to 6h after the tail fin amputation by ANDOR CSU-W1 confocal spinning disk on an inverted NIKON microscope (Ti Eclipse) with ANDOR Neo sCMOS camera (20x air/NA 0.75 objective, mosaic imaging 6x1 with 10% overlap between windows). &nbsp;Laser excitation/emission wavelength: &nbsp;488/521 nm. Time step is 4min (75 repeats). Z-stack is 132&mu;m with &nbsp;4&mu;m step (33 steps). Maximum projection is shown as well. Pixel size is 0.326&mu;m.</p> <p>Macrophages expressing green fluorescent protein Kaede in the cytoplasm are moving toward the wound made at the tail fin.</p>