Raw diffraction images of heliorhodopsin
<p>Crystal structure of heliorhodopsin from <em>Thermoplasmatales archaeon </em>(PDB code: <a href="https://www.rcsb.org/structure/6IS6">6IS6</a>).</p> <p>Small-wedge (10-15°/crystal) datasets collected from loop-harvested microcrystals using <a href="https://github.com/keitaroyam/yamtbx/blob/master/doc/eiger-en.md">EIGER</a> X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm<sup>2</sup> and oscillation step was 0.1°. The crystals belonged to space group C222<sub>1</sub> with unit cell parameters a~52, b~110, c~108 Å.</p> <p>In total 212 small-wedge and two helical (180 & 120°) datasets were collected, and of these 179 datasets were indexed and integrated using XDS with <a href="https://github.com/keitaroyam/yamtbx/blob/master/doc/kamo-en.md">KAMO</a> pipeline. Finally 107 integrated results were merged at 2.4 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result (Shihoya et al. Nature, 2019).</p> <p>This upload also includes SeMet-labeled heliorhodopsin data (not actually used for phasing) and a different crystal form (tetragonal). SeMet data could be merged in P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub> space group at 3.8 Å resolution. The tetragonal data are at 2.8 Å resolution.</p>
ShareScore
40/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 8
- Harmonization
- 4
- Access
- 20
- Reuse readiness
- 8
- Engagement
- 0