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Raw diffraction images of heliorhodopsin

<p>Crystal structure of heliorhodopsin from <em>Thermoplasmatales archaeon </em>(PDB code:&nbsp;<a href="https://www.rcsb.org/structure/6IS6">6IS6</a>).</p> <p>Small-wedge (10-15&deg;/crystal) datasets collected from loop-harvested&nbsp;microcrystals using&nbsp;<a href="https://github.com/keitaroyam/yamtbx/blob/master/doc/eiger-en.md">EIGER</a>&nbsp;X 9M detector at a wavelength of 1 &Aring; on&nbsp;BL32XU, SPring-8. Beam size was around 15&times;10 &micro;m<sup>2</sup>&nbsp;and oscillation step&nbsp;was 0.1&deg;. The crystals belonged to space group C222<sub>1</sub> with unit cell&nbsp;parameters a~52, b~110, c~108 &Aring;.</p> <p>In total 212 small-wedge and two helical (180 &amp; 120&deg;) datasets were collected, and of these 179&nbsp;datasets were&nbsp;indexed and integrated using XDS with&nbsp;<a href="https://github.com/keitaroyam/yamtbx/blob/master/doc/kamo-en.md">KAMO</a>&nbsp;pipeline. Finally 107 integrated&nbsp;results were&nbsp;merged at 2.4&nbsp;&Aring;&nbsp;resolution after CC-based clustering and outlier rejection by KAMO in&nbsp;the published result (Shihoya et al. Nature, 2019).</p> <p>This upload also includes SeMet-labeled heliorhodopsin data (not actually used for phasing) and a different crystal form (tetragonal). SeMet data could be merged in&nbsp;P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub> space group at 3.8 &Aring; resolution. The tetragonal data are at 2.8 &Aring; resolution.</p>

ShareScore

40/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
8
Harmonization
4
Access
20
Reuse readiness
8
Engagement
0

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