Fig. 1 in 2.4.6-Trichloroanisole is a potent suppressor of olfactory signal transduction

Fig. 1. Suppression by TCA of cAMP-induced current. (A) Experimental scheme: whole-cell recording under voltage-clamp (holding potential of −50 mV in all experiments). AC, adenylyl cyclase; Golf, G protein; R, olfactory receptor protein. TCA was included in the puffer pipette at the concentration indicated in each panel, and applied to the cell by pressure ejection (50 kPa). Tip opening diameter of stimulus pipette was 1μm. Current suppression by 10 nM (B), 100 nM (c), 1 μM (D), and 10 μM (E) TCA. Control, black; drug, red; recovery, blue. Data were obtained from different cells. (F) Relation between TCA concentration and SR. Note that the concentrations indicated are the values in the puffer pipette, and the actual concentration at the cell must be much lower than these values. Numbers in parentheses indicate cells examined. Each plot shows the average value and SD.