Screen of A6,B7,1G4 TCRs against a library of HLA-A*02:01 MHC-I peptides from the human exome

<p>T2 cells expressing a library of off targets (derived from A6 and B7 binding motifs in Hausmann 1999) are co-cultured with A6, B7 or1G4 expressing T cells (from a non-A2 donor). Minigenes from surviving cells are amplified and sequenced.</p>