Example data for "A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging"

<p>This project is described in: A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging (2019) and can be found on BioRxiv:&nbsp;<a href="https://www.biorxiv.org/content/10.1101/716787v1">https://www.biorxiv.org/content/10.1101/716787v1</a> and at Nature Methods:&nbsp;<a href="https://www.nature.com/articles/s41592-020-0782-3">https://www.nature.com/articles/s41592-020-0782-3</a></p> <p>Here, we provide example raw datasets of the DNA FluoroCubes with six dyes, Single Dye Cubes, one dye double-stranded DNA constructs, single, biotinylated dyes, and Compact Cube used to determine the photophysical properties.</p> <p>In the zip folder you will find the following datasets with raw, example total internal reflection fluorescence (TIRF) microscopy movies:</p> <p>- FluoroCube_Movies which includes the following movies:<br> &nbsp;&nbsp; &nbsp;- ATTO-488_FluoroCube<br> &nbsp;&nbsp; &nbsp;- ATTO-565_FluoroCube<br> &nbsp;&nbsp; &nbsp;- ATTO-647N_FluoroCube<br> &nbsp;&nbsp; &nbsp;- Cy3_FluoroCube<br> &nbsp;&nbsp; &nbsp;- Cy3N_FluoroCube<br> &nbsp;&nbsp; &nbsp;- Cy5_FluoroCube<br> &nbsp;&nbsp; &nbsp;<br> - Single-Dye-Cube_AND_dsDNA_Movies which includes the following movies:<br> &nbsp;&nbsp; &nbsp;- ATTO-488_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-488_Single-Dye-Cube_single-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-565_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-565_Single-Dye-Cube_single-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-647N_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-647N_Single-Dye-Cube_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3_Single-Dye-Cube_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3N_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3N_Single-Dye-Cube_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy5_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy5_Single-Dye-Cube_single-dye<br> &nbsp;&nbsp; &nbsp;<br> - Single-biotinylated-dye_AND_dsDNA_Movies which includes the following movies:<br> &nbsp;&nbsp; &nbsp;- ATTO-488_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-488_single-biotinylated-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-647N_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- ATTO-647N_single-biotinylated-dye<br> &nbsp;&nbsp; &nbsp;- Cy3_dsDNA_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3_single-biotinylated-dye<br> &nbsp;&nbsp; &nbsp;<br> - Cy3N_Single-Dye-Cube_all-positions_Movies which includes the following movies:<br> &nbsp;&nbsp; &nbsp;- Cy3N_Single-Dye-Cube_SDC-01_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3N_Single-Dye-Cube_SDC-02_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3N_Single-Dye-Cube_SDC-03_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3N_Single-Dye-Cube_SDC-04_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3N_Single-Dye-Cube_SDC-05_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3N_Single-Dye-Cube_SDC-06_single-dye<br> &nbsp;&nbsp; &nbsp;<br> - Compact-Cube_Movies which includes the following movies:<br> &nbsp;&nbsp; &nbsp;- Cy3_FluoroCube_six-dyes<br> &nbsp;&nbsp; &nbsp;- Cy3_Single-Dye-Cube_single-dye<br> &nbsp;&nbsp; &nbsp;- Cy3_Compact-Cube_six-dyes<br> &nbsp;&nbsp; &nbsp;- Cy3_Compact-Cube_single-dye</p>