Figs 3–8 in Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the Beet Webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia

Figs 3–8. Electron microscopy of Tubulinosema loxostegi. 3 – the sporoblast with tubules on its surface; 4 – exospore of the immature spore with short spiky extensions (arrow); 5 – mature spore within the cytoplasm of the host cell showing the disposition of bipartite polaroplast, the polar tube and the nucleus; 6 – the anterior part of the spore demonstrating structure of the anchoring disc and the polaroplast, as well as the additional layer of the exospore (arrow); 7 – the polar tube coils, with posterior coils of lesser diameter; 8 – two spores showing the diplokaryotic arrangement of the nuclei and delamination of the outer layer of the exospore (arrow), possibly due to an artifact of poor tissue preservation. AD – anchoring disc, En – endospore, ER – endoplasmatic reticulum, Ex – exospore, N – nuclei, Pp1 – anterior part of the polaroplast, Pp2 – posterior part of the polaroplast, PT – polar tube, PT1 – anterior coils of PT, PT2 – posterior coils of PT, Tb – tubules. Scale bars: 1 µm (3, 5, 8), 0.5 µm (4, 6, 7).