FIGURE 10 in Evidence on the origin of Narcissus ×weickertii (Amaryllidaceae), an endemic daffodil of the SW Iberian Peninsula

FIGURE 10. In situ hybridization results. A–B: Genomic in situ hybridization (GISH) in three metaphase chromosome spreads of N. ×weickertii (2n= 2x = 18) using DNA from N. papyraceus (detected with biotin) and N. gaditanus (detected with digoxigenin), respectively, as probes. A, GISH in a cell with chromosomes counterstained with DAPI (11 fuchsia-coloured chromosomes from N. papyraceus and 7 blue coloured chromosomes from N. gaditanus); B, GISH in a cell with chromosomes not counterstained with DAPI (11 orange-coloured chromosomes from N. papyraceus and 7 green coloured chromosomes from N. gaditanus. C: Fluorescence in situ hybridization (FISH) in a metaphase cell of N. ×weickertii using pTa71 (18S-5.8S-26S rDNA) as probe (detected with biotin), showing NOR regions in three chromosomes on the left (note that the two red marks up on the right correspond to chromosomes of a different cell). D: Two karyograms obtained from two different cells of N. × weickertii, distinguishing chromosomes of N. papyraceus and N. gaditanus; above, cell treated with GISH (as indicated in A); below, cell treated with FISH (as indicated in C) after GISH followed by reprobing. Scale bar: 10 µm.