Figure 4 in A revised taxonomy and phylogeny of opalinids (Stramenopiles: Opalinata) inferred from the analysis of complete nuclear ribosomal DNA genes

Figure 4. Opalinata subtree showing the results of the TimeTree analysis inferred by applying the RelTime method to the SSU rDNA phylogenetic tree calculated by the maximum parsimony method. Three sets of calibrations including a total of seven time points were combined to obtain the TimeTree (set A* 'sequence evolution'* included three calibration points with uniform distributions; set B* 'host class constraints'* included two maximum time calibration points; and set C* 'host family constraints'* included two maximum time calibration points; see main text for further details); diamonds indicate calibration points included within the Opalinata subtree. Divergence time estimates and their 95% credibility intervals (magenta bars) are indicated in each node. Images showing the evolution of continents are from Scotese (2016).