Figure 4 in Earthworm, a novel in vivo system to validate antimitotic compounds

Figure 4. Immunohistochemistry study with mitosis-specific antibodies: Panels A, B, and C show the analysis of worms treated with water, colchicine, or aqueous extract of rhizomes, respectively, and tested with PCNA antibody. A) High number of PCNA positive cells is shown with 40× lens. Lower number of PCNA-positive cells is shown in panels B and C. Panels D, E, and F show the analysis of worms treated with water, colchicine, or aqueous extract of rhizomes respectively, treated with phospho serine 10 histone H3 antibody. D) A low number of histone H3-positive cells is shown with 40× magnification. A high number of H3-positive cells is shown in panels E and F. Quantitative analysis of positive cells with PCNA (G) and histone H3 (H) cells are shown with mean ± SEM bar and P value (**:P <0.05). Scale bar equals 50 µm.