Figure 2 in Earthworm, a novel in vivo system to validate antimitotic compounds

Figure 2. Inhibition of blastema development in Eudrilus eugeniae by colchicine and aqueous extract of Acorus calamus. A) The adult earthworm, E. eugeniae, is marked to show anterior (ar), clitellum (cl), and posterior (pr) regions. The control worms were injected with distilled water every 24 h for a period of 7 days, and the development of the blastema was observed after day 3 (B), day 5 (C), or day 7 (D). Colchicine was injected similarly for 7 consecutive days and the development of blastema was not observed after day 3 (E), day 5 (F). or day 7 (G). Aqueous extract of A. calamus rhizomes was injected every 24 h for a period of 7 days and the development of blastema was not observed after day 3 (H), day 5 (I), or day 7 (J). rb - regenerating blastema, wh - wound healing.