Figure 1 in mir-331 negatively regulates thyroglobulin secretion via ERp29

Figure 1. Gene expression of endoplasmic reticulum (ER) chaperones and Tg secretion by ERp29 overexpression. (A) In PCCL3 and ERp29over PCCL3 cells, gene expression was estimated for both ER chaperones and ER stress sensors using reverse-transcription polymerase chain reaction (RT-PCR). (B) ER chaperone protein expression was measured using western blotting. (C) Tg secretion by ER29 was measured using western blotting. (D) Tg secretion was measured using tunicamycin treatment followed by western blotting. All experimental conditions are described in detail in the text. Data represent means of at least 3 independent experiments.