FIG. 3 in Hidden Diversity in the Mountain Chorus Frog (Pseudacris brachyphona) and the Diagnosis of a New Species of Chorus Frog in the Southeastern United States

FIG. 3. Genetic clustering based on 21,702 SNPs from AHE loci for P. brachyphona. (A) The Structure plot shows two genetic clusters: Northern (n ¼ 17, green) and Southern (n ¼ 16, blue) as observed in the map (B). The pie charts correspond to the proportion of admixture as estimated in Structure. A blue arrow marks the samples from Hale County, Alabama. Two individuals assigned to the Northern cluster showed introgression from P. feriarum (''out,'' gray). (C) The discriminant analysis of principal components (DAPC) required one discriminant function to explain 67.4% of the genetic variance. Lines at the bottom of the distributions represent one individual. The DAPC also shows two clusters in agreement with the Northern and Southern clusters from Structure. The location of the Tennessee River is shown.