FIG. 3 in Sex-Dependent Cold Tolerance at the Northern Invasive Range Limit of Gambusia affinis on Cape Cod, Massachusetts

FIG. 3. Lower (left) and upper (right) reported thermal tolerances for Gambusia affinis and Gambusia holbrooki. Each point and line represent the mean and range, respectively, for a particular population, with lethal and non-lethal assays distinguished. Significant variation in reported values for a given population is typically due to varied acclimation temperatures and separate testing on males and females. Gray lines show extrapolated 50% lower thermal tolerance predictions and standard errors for average males and females from our study. Letters indicate sexes included, and numbers refer to studies in Table S1 (see Data Accessibility).