Fig. 1 in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation

Fig. 1. Histopathological lesions associated with endogenous stages of Eimeria spp. in sections of the ileum and colon of European bison (H-E staining). (A) Shortening and blunting of the intestinal villi of the ileum with diffuse infiltration of mononuclear inflammatory cells within the lamina propria, edematous stroma, dilated crypt containing necrotic debris (arrow), and atrophy of submucosal lymphoid follicles (× 20 magnification). (B) Schizonts and degenerating merozoites in the crypt lumen of the colon (arrows); immature macrogamont with a central nucleus (arrowhead) (× 1000 magnification). (C) Immature microgamonts in the epithelial cells of the colon crypt (arrows) (× 400 magnification). (D) Mature microgamont in the epithelial cells of the colon crypt (arrow) (× 1000 magnification). (E) Gametogonic stages of Eimeria development in the epithelial cells of the colon. Microgamont with peripheral microgames (arrowhead), (a) nearly mature microgamonts, (b) macrogamont with eosinophilic wall-forming bodies, (c) early oocyst (× 400 magnification). (F) Mature macrogamont in the epithelial cells of the cecum (arrow) (× 1000 magnification).