Fig. 2 in Toxoplasma gondii infection in European mouflons (Ovis musimon) and captive wild felines from Puebla, M�exico

Fig. 2. Representative PCR-RFLP pattern of T. gondii for SAG3 gene. Single and mixed infections in lions and mouflons tissues were observed. A. PCR for the SAG3 locus of lion 1 (L1, spleen), mouflon 1 (M1, brain) and mouflon 2 (M2, liver) samples. B. A triple infection is highlighted (yellow box). Resulting genotypes are specified at the bottom. In silico digestion was done by www.benchling.com. Reference strains sequences GT1, TGGT1_308020; Me49, TGME49_308020; VEG, TGVEG_308020 are available at www.toxodb.org. MW: molecular weight marker; RH and ME49 are reference strains, type I and II, respectively; M: mouflon, L: lion. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)