Fig. 1 in Toxoplasma gondii infection in European mouflons (Ovis musimon) and captive wild felines from Puebla, M�exico

Fig. 1. Toxoplasma gondii infection in captive mouflons in 2011 and 2012. A. Anti-T. gondii frequency distribution in fifty-five sera of mouflons sampled in 2011; black arrows indicate positive samples as determined by their position to the right of the normally distributed values of the left population. B. One year later, 41/55 original mouflons were captured, bled, and re-tested for antibodies against this parasite. The cut-off point used was 1.0 RI (dotted lines), which separated the negative population (left) from the positive cases. C. Three mouflons remained positive, five became negative, and four seroconverted positive in 2012 (red, green and blue dots, respectively); one mouflon is on the cut-off (orange dot). D. Immunohistochemistry for T. gondii in tissues from in the spleen of mouflon 1, where an immunopositive cumulus of tachyzoites can be seen (blue arrow). The nuclei of resident lymphocytes and dendritic cells were contrasted with Meyer's hematoxylin. Bar: 50 μm. R = Pearson correlation. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)