Fig. 3 in P e r s p e c t i v e Integrating current methods for the preservation of amphibian genetic resources and viable tissues to achieve best practices for species conservation

Fig. 3. The "tissue piecing" protocol used to preserve viable cells for establishment of cell lines in the future. A) Tissue is cut into long, thin strips. B) Tissue is diced into 1 mm3 fragments before adding medium containing 10% DMSO as a cryoprotectant. C) Prepared tissue is stored in LN2 until future cell culture is possible; those without cell culture capability can transport samples using a dry shipper to maintain cold-chain.