Fig. 1 in Workflow of Lotmaria passim isolation: Experimental infection with a low-passage strain causes higher honeybee mortality rates than the PRA-403 reference strain

Fig. 1. Workflow for the isolation of bee-infecting trypanosomatid parasites from honeybee guts. A. Dissection and tissue processing (steps 1–4), and trypanosomatid culture and expansion (step 5) in liquid or Solid Cultures. B. Growth curve of L. passim PRA-403 strain in decreasing concentrations of 5-Fluorocytosine (1 × 106 μg/ mL-100 μg/mL) to determine the maximum dose for parasite survival. C. Giemsa staining of L. passim C1 (CCP 1). D. Hoescht DNA staining of live L. passim C1 (CCP 1): N, Nucleus; K, Kinetoplast; E, Scanning Electron Microscopy of L. passim C1 (CCP 1) grown in Agar Solid cultures 20 days post-inoculation.