Figure 4 in Protandric simultaneous hermaphroditism in Salmoneus carvachoi Anker, 2007 (Decapoda: Alpheidae): a new sexual system in alpheid shrimps

Figure 4. Transition between male and female reproductive system of Salmoneus carvachoi* A, gross morphology of the distal vas deferens region (DVD)* Slight dilated ampoule shows the androgenic gland (arrow)* B, detail of the androgenic gland from a hermaphrodite phase individual positioned in the same place as the male phase individuals* C, light microscopy of DVD slightly wider than the MVD* D, electron micrograph of DVD with the typhlosole as a discrete salient fold on one side of the vas deferens* E, histochemical aspect of DVD seminal fluid with the secretion type I without acid polysaccharides as well the globular compound of the secretion type II (white arrow)* The homogeneous compound of secretion type II is reactive to Alcian blue stain (black arrow)* F, fractured DVD showing the small amount of seminal fluid with spermatozoa immersed in the secretion type I surrounded by the secretion type II (arrow)* G, detail of the spermatozoon with tack morphology and a long spike* The secretion type I shows small granules, thin fibrils and some larger droplets (arrow)* H, thick musculature of the ampoule with many muscular fibres* The primordial spermatophore shows small amount of seminal fluid of the secretion type I and is surrounded by a thin layer of secretion type II (arrow)* I, male phase individual showing ovaries and testes forming the ovotestes, surrounded by blood capillaries (arrow)* The ovaries are filled with oogonia forming the germinal centre at the inner periphery of the ovarian lobe close the testes* The primary oocytes occupy the rest of the lobule while the spermatogenesis is still producing spermatozoa (arrowhead)* J, detail of the oogonia and primary oocytes arrested in the previtellogenic stage surrounded by follicle cells (black arrowhead)* Notice primary spermatocytes and spermatozoa (white arrowhead) in the testes* K, primordial ovaries found in male phase individuals* The ovarian wall cells are arranged in different strata around the ovary lumen forming a mandibulate type ovary* Detail of the ovarian wall cells showing long microvilli (arrowhead)* These cells are laying on the connective tissue shared with the testes* I–L, haematoxylin and eosin stain* M, ovarian wall cells with nucleus with mitotic prophase chromosome (arrow heads)* Toluidine blue stain* A *