Figure 2 in Protandric simultaneous hermaphroditism in Salmoneus carvachoi Anker, 2007 (Decapoda: Alpheidae): a new sexual system in alpheid shrimps

Figure 2. Male reproductive system of Salmoneus carvachoi* A, general view showing the small testes (TE) and thin vasa deferentia (VD)* B, detail of the testes (TE) showing the lobular anatomy* Each lobule depicts 'Y-shaped' morphology* C, histology of the testes (TE) classified as lobular (acinous) type with each lobule (SL) filled with cells in the same stage of spermatogenesis* The spermatozoa (arrow) are released into seminiferous duct connected to the proximal vas deferens (PVDa)* Haematoxylin and eosin stain* D, detail of the seminiferous lobules (SL) filled with primary spermatocytes and another with metaphasic plates and anaphasis of meiosis I (arrow heads)* Each lobule is surrounded with accessory cells* Haematoxylin and eosin stain* E, F, longitudinal and transversal section, respectively, of the spermatozoon showing the spike (white arrowhead) and the acrosomal cap more basophilic (black arrowhead) above the nucleus* Haematoxylin and eosin stain* Scale bar = 4 um* G, spermatozoon reactive to proteins in the spike (white arrowhead) and strongly positive at the acrosomal cap (black arrowhead)* Xylidine ponceau stain* Scale bar = 4 um* H, absence of reaction to neutral polysaccharides in the spermatozoon* PAS stain* Scale bar = 4 um* I, ultrastructure of the testes and its continuity with the PVD* The anterior part of PVD running from the testes above the vas deferens and emerge at the centre of the coiled structure that compose the main part of PVD region which opens in the straight medium vas deferens (MVD)* J, detail of the PVD showing the anterior part emerging from the centre, whereas the distal part is coiled and showing the flap of typhlosole (arrow)* K, light microscopy of PVD with the anterior part filled with spermatozoa immersed in basophilic secretion* In the coiled posterior part of the PVD the sperm mass is packed against one side of the lumen in opposition to typhlosole (arrow)* Haematoxylin and eosin stain* AC, accessory (Sertoli) cell; EP, epithelium; L, testes lobule; MVD, medium vas deferens region; PVD, proximal vas deferens region; PVDa, anterior part of proximal vas deferens; PVDp, posterior part of proximal vas deferens; SCI, primary spermatocytes in meiotic prophase; SL, seminiferous lobules; T, typhlosole; TE, testes; VD, vas deferens*