Figure 5 in Protandric simultaneous hermaphroditism in Salmoneus carvachoi Anker, 2007 (Decapoda: Alpheidae): a new sexual system in alpheid shrimps

Figure 5. Female reproductive system of a hermaphrodite individual of Salmoneus carvachoi* A, general view of ovotestes showing the small testes and thin vasa deferentia compared to the ovarian portion* The ovaries are organized in two lobes that grow anteriorly while the oviducts are positioned more posteriorly* B, light microscopy of the ovotestes* The female portion is voluminous showing vitellogenic oocytes surrounded with follicle cells* Notice the oviducts are in a more posterior position* The small male part is posterior and marked by the coiled proximal vas deferens* Haematoxylin and eosin stain* C, detail of ovotestes' connective tissue shared by the ovarian portion and testes portion (black arrow)* The anterior part of the proximal vas deferens is filled with spermatozoa* The ovaries have oocytes in both exogenous and endogenous vitellogenesis* Haematoxylin and eosin stain* D, SEM of the ovarian lobes showing the germinal centre as an inner shaft of small cells in each lobule at the mid-dorsal region of the cephalothorax (arrow)* The large vitellogenic oocytes are externally positioned in the lobe* E, detail of the germinal centre and connective tissue with many fenestrations (arrowheads)* F, vitellogenic oocytes with blood capillaries with haemocytes inside (arrowhead)* Notice in a fractured area the oolemma (white arrow) and the cells surface of the follicle cell (black arrow)* G, detail of ovarian follicle surface (arrow) and the haemocyte in the blood capillary (arrowhead)* H, endogenous vitellogenesis oocyte with small cytoplasm vesicles reactive to neutral polysaccharides and a few lipid droplets* The exogenous vitellogenic oocytes show mature yolk granules also positive to PAS stain* I, histology of the germinal centre with oogonia and their chromosomes in mitotic prophase* The pre-vitellogenenic oocyte has homogeneous basophilic cytoplasm* The ovaries show blood capillaries with haemocytes (arrow)* J, positive reaction for proteins in the yolk granules (arrow) in a mature oocyte also filled with numerous lipid droplets* The previtellogenic oocytes have homogeneous reaction in the cytoplasm* K, detail of the closed oviduct–ovary region (white arrow)* The oviduct is a simple cubic epithelium with closed lumen (black arrow)* Notice the numerous haemocytes in the blood capillary* C, capillary; CT, connective tissue; EO, Endogenous vitellogenic primary oocyte F, follicle cell; GC, germinal centre; HE, hemocyte; LI, lipid droplet; OC, exogenous vitellogenic primary oocyte; OD, oviduct; OF, ovarian follicle; OL, ovarian lobe; OO, oogonia; OT, ovotestes; OV, ovary; PO, previtellogenic oocyte; PVD, proximal vas deferens; TE, testes; VD, vas deferens*