HPTLC Data of "Metal Ion Cofactors Modulate Integral Enzyme Activity By Varying Differential Membrane Curvature Stress"

<p>Lipid hydrolysis by the integral membrane protein OmpLA (outer membrane phospholipase). The enzymatic degradation of the proteoliposomes was determined by TLC. After lipid extraction against organic solvent (2:1 vol/vol chloroform/methanol) based on the Folch extraction method, the samples were spotted on a silica plate (Sigma-Aldrich, Steinheim, Germany) with the automatic TLC sampler 4 (CAMAG, Muttenz, Switzerland). The mobile phase in the developing chamber was a solvent mixture composed of 32.5:12.5:2 vol/vol/vol CHCl_3/MeOH/H_2O. After drying, the plate was immersed in a developing bath (5.08 g MnCl_2 dissolved in 480 ml H_2O, 480 ml EtOH and 32 ml H_2SO_4), which is sensitive to double bonds, and dried for 15 min at 120&deg;C. To quantify the lipid concentrations the plate was scanned with the TLC scanner 3 (CAMAG, Muttenz, Switzerland) and further analyzed with WinCats software.</p> <p>Lipids:</p> <ul> <li>1-palmitoyl-2-oleoyl-<em>sn</em>-glycero-3-phosphocholine (POPC)</li> <li>1-palmitoyl-2-oleoyl-<em>sn</em>-glycero-3-phosphoethanolamine (POPE)</li> <li>1-palmitoyl-2-oleoyl-<em>sn</em>-glycero-3-phosphoglycerol (POPG)</li> </ul>