FIGURE 3 in Age-associated hemogram and ultrastructural leukocyte morphology in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Brazilian Pantanal

FIGURE 3 | Neutrophil and PAS-positive granular leukocytes in Pygocentrus nattereri from Brazilian Pantanal in light (A and D) and transmission electronic microscopy (B, C, E, F). A–C. Neutrophil with a lumpy nucleus and cytoplasmic vacuoles showing fine azurophilic and neutrophilic granulations. B. Mitochondria (m) and an extensive network of cell synthesis organelles are also observed; nucleus (n); C, vacuoles (v) and granules with varying size and electron density are observed. D–F. PAS-positive granular leukocyte with a displaced euchromatic nucleus; cytoplasm rich in small vacuoles and azurophilic granulation. In E and F, the granules content electrolytic material is observed (g), possibly with internal electrodense areas in an elongated half-moon shape (arrows). Scale bars = A and D = 10 µm (May Grunwald-Giemsa-Wright staining), B and E = 2 µm; C and F = 500 nm.