Figure 3. A in Expression analysis of phosphate induced genes in contrasting maize genotypes for phosphorus use efficiency

Figure 3. A – Northern blot analysis of phosphate starvation-induced ZmPTs genes in maize genotypes. B – Expression of ZmPTs in a plant grown in different phosphorus concentrations. C – Suppression of the ZmPTs expression by Pi resupply. D – Expression of ZmPT genes using RNA isolated from different root parts. E – Expression of ZmPTs in different maize plants under Pi starvation. F – Effect of duration of phosphate starvation on ZmPTs genes expression in maize genotypes. G – Expression of ZmPT homologs in roots of two sorghum genotypes. Total RNA isolated from different times of hydroponically grown plants supplied with half-strength modified Hoagland's solution containing 250 µM phosphate (+) or no phosphate (-) for different days or different concentrations as indicated. All the blots were probed with 32P labeled ZmPTs. The panel below the Northern blots is the ethidium bromide-stained gel prior to blotting showing the RNA integrity and uniformity of loading.