Gene expression profiling of normothermic machine perfusion of human livers

<p>Normothermic machine perfusion (NMP) has been successfully implemented in clinical routine of liver transplantation over the past years. However, little is known about the mechanisms how NMP impacts on the transcriptome of a human donor liver. We herein examined gene expression profiles in transplanted and non-transplanted livers over NMP time. 50 livers subjected to NMP were included in this study. 30 were transplanted after a maximum of 20 hours (h) perfusion, while 15 were discarded due to poor performance. Biopsies were collected before NP (PRE), 1h, 6h, 12h, 20h of NMP and after reperfusion. Next-generation sequencing was applied in liver biopsies to assess differential gene expression over perfusion time. Perfusate samples were collected regularly to monitor liver function. Significantly differentially expressed genes between each timepoint and PRE (LIVER_NMP_TIMEPOINT_PRE_DEG.xlsx) as well as between non-transplanted livers (NTP) and transplanted livers (TP) (LIVER_NMP_NTP_TP_DEG.xlsx) were determined using the R package DESeq2 and included in this dataset.</p>