FIGURE 7 in Two new green algae, Rhizoclonium fractum sp. nov. and R. umbraticum sp. nov., from tropical and subtropical brackish waters of Japan

FIGURE 7. Field-collected specimens of Cladophoraceae sp. 2 (Rhizoclonium minutissimum). (A) Field-collected specimens. (B, C) Morphology of field-collected specimens. (D, E) Filamentous thalli with non-septate rhizoids. (F) Tip of filamentous thalli. (G–I) Spindle- and band-shaped chloroplasts. Arrowhead points to a pyrenoid. (J) Autofluorescence of chloroplasts. (K) Cells stained with DAPI, showing nuclei. (L) Cells stained with Lugol's iodine, showing pyrenoids. (M, N) Pyrenoids observed by transmission electron microscopy. Pyrenoids have starch plates. (M) Three or two thylakoid membranes traversing a pyrenoid. (33) One thylakoid membrane. Scale bar = 5 mm (A); Scale bars = 40 μm (B, C); Scale bars = 20 μm (D–G, K, L); Scale bars = 10 μm (H–J); Scale bars = 0.5 μm (M); Scale bars = 1 μm (N).