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Cilia structure and intraflagellar transport differentially regulate sensory response dynamics within and between C. elegans chemosensory neurons

<p><u>List of data with corresponding files</u></p> <p><strong>Figure 1</strong></p> <p>A)&nbsp;&nbsp;&nbsp; <em>File name:</em> Figure 1A- ASH_IFT_mutants_length_copy.pzfx</p> <p>B)&nbsp;&nbsp;&nbsp; <em>File names: </em>(Folder) ASH_1Mglycerol_IFT_mutants <em>and </em>(Folder) ASH_10-2IAA_IFT_mutants</p> <p>C)&nbsp;&nbsp;&nbsp; <em>File names: </em>(Folder) ASH_1Mglycerol_IFT_mutants &gt; Peak_responses <em>and </em>(Folder) ASH_10-2IAA_IFT_mutants &gt; Peak_responses - first pulse</p> <p>D)&nbsp;&nbsp; <em>File names: </em>Figure 1D &ndash; ASH Cher and Marianas Lengths Truncation osm-3(ts) 27 and 30 degree graph <em>and </em>Figure 1D &ndash; ASH Cher and Marianas Lengths Truncation osm-3(ts) 27 degrees</p> <p>E)&nbsp;&nbsp;&nbsp; <em>File name: </em>Figure 1E &ndash; ASH IFT Quantification.xlsx</p> <p>F)&nbsp;&nbsp;&nbsp; <em>File name: </em>(Folder) ASH_1Mglycerol_osm3ts</p> <p>G)&nbsp;&nbsp; <em>File names: </em>Figure 1 &ndash; Glycerol_Assays_Data.pzfx <em>and </em>Figure 1 &ndash; Glycerol_Assays_Data.xlsx</p> <p>H)&nbsp;&nbsp; <em>File name: </em>Figure 1H &ndash; ALL ASH Marianas and Cher Lengths che-3(nx159ts)</p> <p>I)&nbsp;&nbsp;&nbsp;&nbsp; <em>File names: </em>Figure 1- ASH IFT PY12007 v AP457 <em>and </em>Figure 1- ASH IFT PY12007 v AP457.xlsx</p> <p>J)&nbsp;&nbsp;&nbsp; <em>File name:</em> (Folder) ASH_1Mglycerol_che3ts</p> <p>K)&nbsp;&nbsp; <em>File names: </em>Figure 1 &ndash; Glycerol_Assays_Data.pzfx <em>and </em>Figure 1 &ndash; Glycerol_Assays_Data.xlsx</p> <p><strong>Figure 2</strong></p> <p>B) &nbsp;<em>File name: </em>(Folder) 10-7Diacetyl_Calcium_IFT_mutants</p> <p>C)&nbsp; <em>File names: </em>(Folder) 10-6Pyrazine_Calcium_IFT_mutants <em>and </em>(Folder) 10-7Pyrazine_Calcium_IFT_mutants</p> <p>D)&nbsp;<em>&nbsp;File names: </em>(Folder) Microfluidics &gt; (Folder) 10-6pyrazine <em>and </em>(Folder) 10-7diacetyl</p> <p>E)&nbsp; <em>File names: </em>(Folder) Microfluidics &gt; Diacetyl_Chemotaxis_index_osm6_osm3kap1.pzfx&nbsp;<em>and</em><em>&nbsp;</em>Pyrazine_Chemotaxis_index_pyrazine_osm3kap1_osm6.pzfx</p> <p>G)&nbsp; <em>File name: </em>(Folder) 10-6Pyrazine_Calcium_misexpression</p> <p><strong>Figure 3</strong></p> <p>A)&nbsp;&nbsp; <em>File name: </em>Figure 3A- AWA IFT AP303 v AP379.xlsx</p> <p>C)&nbsp; <em>File names: </em>(Folder) AWA_Calcium_Diacetyl <em>and </em>(Folder) AWA_Calcium_Pyrazine</p> <p>D) &nbsp;<em>File names: </em>(Folder) AWA_Calcium_Diacetyl <em>and </em>(Folder) AWA_Calcium_Pyrazine</p> <p>E)&nbsp; <em>File name: </em>Figure 3E- SRX64 and ODR10 within dendritic branches.pzfx</p> <p><strong>Figure 4</strong></p> <p>A)&nbsp;&nbsp; <em>File name: </em>(Folder) AWA_Calcium_Diacetyl_grk2</p> <p>B)&nbsp;&nbsp; <em>File name: </em>(Folder) AWA_Calcium_Pyrazine_grk2</p> <p>C)&nbsp;&nbsp; <em>File name: </em>(Folder) Microfluidics_diacetyl</p> <p>D)&nbsp;&nbsp; <em>File name: </em>(Folder) Microfluidics_pyrazine</p> <p>E)&nbsp;&nbsp;&nbsp; <em>File name: </em>Figure 4E- GRK2tRFP_kap1osm3ts.pzfx</p> <p><strong>Figure 5</strong></p> <p>A)&nbsp;&nbsp; <em>File names: </em>(Folder) AWA_Diacetyl_Adaptation_osm3ts <em>and </em>(Folder) AWA_Pyrazine_Adaptation_osm3ts</p> <p>B)&nbsp;&nbsp;&nbsp; <em>File name: </em>Figure 5B and S7B- FRAP.pzfx</p> <p>C)&nbsp;&nbsp;&nbsp; <em>File names: </em>Figure 5C- SRX64_bbs7jhu590.pzfx <em>and </em>Figure 5C- SRX64_bbs7ok1351.pzfx <em>and </em>Figure 5C- oy158_bbs7jhu590.pzfx <em>and </em>Figure 5C- oy158_bbs7ok1351.pzfx</p> <p>D)&nbsp;&nbsp; <em>File names: </em>(Folder) AWA_Diacetyl_Adaptation_bbs7 <em>and </em>(Folder) AWA_Pyrazine_Adaptation_bbs7</p> <p>E)&nbsp;&nbsp;&nbsp; <em>File name: </em>Figure 5E- Marianas_EV_ODR10_SRX64_osm3tskap1.pzfx</p> <p><strong>Figure S1</strong></p> <p>A)&nbsp;&nbsp; <em>File names: </em>(Folder) ASH_IFTmutants_0.5and2Mglycerol &gt; (Folder) 0.5Mglycerol <em>and </em>(Folder) 2Mglycerol</p> <p>B)&nbsp;&nbsp;&nbsp; <em>File names: </em>(Folder) ASH_IFTmutants_quinine &gt; (Folder) 5mM_quinine <em>and </em>(Folder) 10mM_quinine</p> <p>C)&nbsp;&nbsp;&nbsp; <em>File name: </em>ASH_IAA_t1/2</p> <p>D)&nbsp;&nbsp; <em>File names: </em>(Folder) ASH_IFTmutants_heptanol &gt; (Folder) 10-3_heptanol <em>and </em>(Folder) 10-4_heptanol</p> <p>E)&nbsp;&nbsp;&nbsp; <em>File names: </em>(Folder) ASH_IFTmutants_2Mglycerol_GABA <em>and </em>(Folder) ASH_IFTmutants_2Mglycerol_bicuculline</p> <p><strong>Figure S2</strong></p> <p>B)&nbsp;&nbsp;&nbsp; <em>File name: </em>Figure S2B- Dye-filling_data</p> <p>C)&nbsp;&nbsp;&nbsp; <em>File name: </em>Figure S2C- ASH_IFT_mutants_length.pzfx</p> <p>D)&nbsp;&nbsp; <em>File names: </em>Figure S2D- ASH IFT PY6345 v PY12007 <em>and </em>Figure S2D- ASH IFT Quantification</p> <p><strong>Figure S3</strong></p> <p>A)&nbsp;&nbsp; <em>File name: </em>(Folder) AWA_10-7Diacetyl_IFTmutants</p> <p>B)&nbsp;&nbsp;&nbsp; <em>File names:</em> (Folder) AWA_Calcium_heptanone &gt; (Folder) 10-4_heptanone <em>and </em>(Folder) 10-5_heptanone</p> <p>C)&nbsp;&nbsp;&nbsp; <em>File name: </em>(Folder) AWA_10-5Pyrazine_IFTmutants</p> <p>D)&nbsp;&nbsp; <em>File names: </em>(Folder) AWA_Calcium_methylpyrazine &gt; (Folder) 10-8_methylpyrazine <em>and </em>(Folder) 10-9_methylpyrazine</p> <p>E)&nbsp;&nbsp;&nbsp; <em>File name:</em> Figure S3- Chemotaxis IFT_mutants.pzfx</p> <p><strong>Figure S4</strong></p> <p>A)&nbsp; <em>File name: </em>Figure S4A &ndash; AP419_pyrazine_Chemotaxis.pzfx</p> <p>C)&nbsp;<em>&nbsp;File name: </em>Figure S4C &ndash; srx64SL2gfp_osm5.pzfx</p> <p><strong>Figure S6</strong></p> <p>A)&nbsp;&nbsp; <em>File names: </em>Diacetyl_prism_decay_grk2 <em>and </em>Pyrazine_prism_decay_grk2</p> <p><strong>Figure S7</strong></p> <p>&nbsp;A)&nbsp; <em>File name: </em>(Folder) AWA_Calcium_Adaptation_diacetyl_IFT_mutant</p> <p>&nbsp;B)&nbsp; <em>File name: </em>Figure 5B and S7B- FRAP</p> <p>&nbsp;C)&nbsp; <em>File name: </em>Figure S7C- oy158_grk2_rescue.pzfx</p> <p>&nbsp;F)&nbsp; <em>File names: </em>(Folder) AWA_Calcium_Adaptation_diacetyl_grk2 &gt; (Folder) grk2 <em>and </em>(Folder)&nbsp;grk2bbs7double</p> <p><strong>Additional materials</strong></p> <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; All code is listed under Code from Github &gt; MF.matR-master <em>and </em>Additional Methods</p>

ShareScore

32/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
4
Harmonization
4
Access
16
Reuse readiness
8
Engagement
0