Structures of phages homing nucleases in complex with Zn and dsDNA

<p><span>Structures were predicted by AlphaFold3 on google servers at&nbsp;<a href="https://golgi.sandbox.google.com" target="_blank" rel="noopener">https://golgi.sandbox.google.com</a> by introducing the sequence of the HNH protein, a Zn⁺⁺ ion and a dsDNA of length between 26-32 base-pairs.</span></p> <p><span>The predicted models report interactions between two residues of HNHs and the </span><span>catalytic </span><span>Zn⁺⁺ ion</span><span> as well as with the dsDNA. The HNHs N-terminal domain fits in a large dsDNA groove, bringing the </span><span>Zn⁺⁺ ion in the vicinity of the dsDNA backbone upper lip of the small groove. Except for HNH3, HNH4, HNH9 and HNH10, the C-terminal domain fits in the following large groove. The HNH3 and 4 C-terminal domain fits on the other side of the dsDNA. HNH9 and 10 do not possess a globular C-terminal domain. Between the two globular domains, a linker helix fits into the small groove between the two large grooves. To note, this linker helix harbours at its N-terminus one of the Zn⁺⁺ ion binding residue.</span></p> <p>&nbsp;</p>