Comparative analyses of compatible and incompatible host-pathogen interactions provide insight into divergent host specialization of closely related pathogens

<p><strong><span>Supplementary Animations</span></strong></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S1. Directed growth of <em>Z. pseudotritici</em> Zp13 hyphae towards wheat stomata 7&nbsp;dpi. </span></strong><span>Tomographic animation of confocal image z-stack showing spore germination, filament development, and penetration of wheat stomata by hyphae of <em>Z. pseudotritici</em> isolate Zp13 at 7 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Scale bar = 50 &micro;m.</span></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S2. Directed growth of <em>Z. ardabiliae </em>Za17 hyphae towards wheat stomata 17&nbsp;dpi. </span></strong><span>Tomographic animation of confocal image z-stack showing spore germination, filament development, and penetration of wheat stomata by hyphae of <em>Z. ardabiliae</em><strong> </strong>isolate Za17 at 17 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Scale bar = 25 &micro;m.</span></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S3. <em>Z. pseudotritici</em> Zp13 hyphae penetrate wheat stoma 14&nbsp;dpi. </span></strong><span>Tomographic animation of confocal image z-stack showing penetration of wheat stoma by two hyphae of <em>Z.&nbsp;pseudotritici</em> isolate Zp13 at 14 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Scale bar = 20 &micro;m.</span></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S4. <em>Z. ardabiliae </em>Za94 hyphae penetrate wheat stoma 8&nbsp;dpi.</span></strong><span> Tomographic animation of confocal image z-stack showing penetration of wheat stoma by two hyphae of <em>Z.&nbsp;ardabiliae</em> isolate Za94 at 8 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Reference transmitted images in <em>grey</em>. Scale bar = 25 &micro;m.</span></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S5.</span></strong><span> <strong><em>Z. pseudotritici </em>Zp72<em> </em>hypha arrested between guard cells 10&nbsp;dpi. </strong>Tomographic animation of confocal image z-stack showing infecting hypha of <em>Z. pseudotritici</em> isolate Zp72 that is arrested at wheat stomatal guard cells at 10 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Reference transmitted images in <em>grey</em>. Scale bar = 25 &micro;m.</span></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S6. <em>Z. ardabiliae </em>Za94<em> </em>hyphae arrested between guard cells 17&nbsp;dpi. </span></strong><span>Tomographic animation of confocal image z-stack showing infecting hyphae of <em>Z.&nbsp;ardabiliae</em> isolate Za94 that are arrested between wheat stomatal guard cells at 17 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Scale bar = 25 &micro;m.</span></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S7. <em>Z. ardabiliae </em>Za48<em> </em>hypha arrested in sub-stomatal cavity 8&nbsp;dpi. </span></strong><span>Tomographic animation of confocal image z-stack showing infecting hypha of <em>Z.&nbsp;ardabiliae</em> isolate Za48 that is arrested in a wheat sub-stomatal cavity at 8 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Reference transmitted images in <em>grey</em>. Scale bar = 25 &micro;m.</span></p> <p><span>&nbsp;</span></p> <p><strong><span>Animation S8. <em>Z. pseudotritici </em>Zp13<em> </em>hypha arrested in sub-stomatal cavity 17&nbsp;dpi. </span></strong><span>Tomographic animation of confocal image z-stack showing infecting hypha of <em>Z. pseudotritici</em> isolate Zp13 that is arrested in a wheat sub-stomatal cavity at 17 dpi. Nuclei and wheat cells displayed in <em>purple</em> and fungal structures in <em>green</em>. Scale bar = 25 &micro;m.</span></p>