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Table 3 in A revised taxonomy and phylogeny of opalinids (Stramenopiles: Opalinata) inferred from the analysis of complete nuclear ribosomal DNA genes

Table 3. List of sequences used in the present study. Sequences lacking>100 bases at the 5 ʹ or 3 ʹ ends are shown in parentheses. New sequences are marked in bold.<table><tbody><tr><th>Sequence/species</th><th>Accession sequence</th></tr><tr><th>Number</th><th>Length</th><th>GC%</th><th>Source</th></tr><tr><th>SSU rDNA</th></tr><tr><th>STRAMENOPILES</th></tr><tr><th>OPALINATA</th></tr><tr><th>Opalinida</th></tr><tr><th>(Protoopalina intestinalis (Stein* 1856) Metcalf* 1923)</th></tr></tbody><tbody><tr><th></th><td>AY576545</td><td>1409</td><td>33.6</td><td>Kostka et al. (2004)</td></tr><tr><th>(Protoopalina axonucleata Metcalf * 1923a)</th><td>AB175929</td><td>1263</td><td>32.5</td><td>Nishi et al. (2005)</td></tr><tr><th>Protoopalina limnocharis</th><td>MN625264</td><td>1829</td><td>32.2</td><td>This study</td></tr><tr><th>Protoopalina pingi</th><td>MN625266</td><td>1830</td><td>32.4</td><td>This study</td></tr><tr><th>Zelleriella orientalis</th><td>MN625263</td><td>2143</td><td>26.4</td><td>This study</td></tr><tr><th>Zelleriella sp.</th><td>MN625265</td><td>2143</td><td>26.4</td><td>This study</td></tr><tr><th>Opalina undulata</th><td>MF434114</td><td>2167</td><td>26.8</td><td>Li et al. (2018)</td></tr><tr><th>Opalina triangulata</th><td>MK872804</td><td>2166</td><td>26.8</td><td>Wang et al. (2019)</td></tr><tr><th>Opalina obtrigonoidea</th><td>MK970648</td><td>2167</td><td>26.8</td><td>Zhao et al. (2020)</td></tr><tr><th>Opalina japonica</th><td>MN625267</td><td>2170</td><td>26.0</td><td>This study</td></tr><tr><th>Opalina longa</th><td>MN625268</td><td>2171</td><td>27.7</td><td>This study</td></tr><tr><th>Proteromonadida</th></tr><tr><th>Karotomorpha sp.</th><td>DQ431242</td><td>1858</td><td>44.1</td><td>Kostka et al. (2007)</td></tr><tr><th>(Karotomorpha sp.)</th><td>DQ431243</td><td>1424</td><td>46.2</td><td>Kostka et al. (2007)</td></tr><tr><th>Proteromonas lacertae Grassé * 1879</th><td>U37108</td><td>1743</td><td>37.4</td><td>Leipe et al. (1996)</td></tr><tr><th>BLASTOCYSTIDA</th></tr><tr><th>Blastocystis cycluri Singh et al. * 1996</th><td>AY266474</td><td>1848</td><td>35.6</td><td>Yoshikawa et al. (2004)</td></tr><tr><th>Blastocystis lapemi Teow et al. * 1991 Blastocystis pythoni Singh et al. * 1996</th><td>AY266471 AY266472</td><td>1840 1794</td><td>36.7 39.1</td><td>Yoshikawa et al. (2004) Yoshikawa et al. (2004)</td></tr><tr><th>Blastocystis hominis (Swaine & Britain* 1849) Brumpt* 1912</th><td></td><td></td><td></td></tr><tr><th>Blastocystis ratti Chen et al. * 1997 PLACIDIDA</th><td>AB023499 AY590114</td><td>1740 1730</td><td>43.6 40.3</td><td>Arisue et al. (2002) Noel et al. (2005)</td></tr><tr><th>Placidia cafeteriopsis Moriya et al. * 2002</th><td>JF834161</td><td>1775</td><td>49.4</td><td>Chan YF* Chen CW* Tsai AY* Chiang KP* unpublished</td></tr><tr><th>Suigetsumonas clinomigrationis Okamura & Kondo * 2015</th><td></td><td></td><td></td></tr><tr><th>Wobblia lunata Moriya et al. * 2000 BICOSOECIDA</th><td>AB976561 AB032606</td><td>1769 1726</td><td>52.0 49.1</td><td>Okamura and Kondo (2015) Moriya et al. (2000)</td></tr><tr><th>Bicosoeca petiolata (Stein* 1878) Prinsheim* 1946</th><td></td><td></td><td></td></tr><tr><th></th><td>AY520444</td><td>1809</td><td>48.6</td><td>Cavalier-Smith and Chao (2006)</td></tr><tr><th>Cafeteria roenbergensis Fenchel & Patterson * 1988</th><td></td><td></td><td></td></tr><tr><th></th><td>AF174364</td><td>1695</td><td>47.1</td><td>Atkins et al. (2000)</td></tr><tr><th>ALVEOLATA</th></tr><tr><th>APICOMPLEXA</th></tr><tr><th>Babesia gibsoni (Patton* 1910)</th><td>KC461261</td><td>1665</td><td>45.5</td><td>Mandal et al. (2015)</td></tr><tr><th>Eimeria necatrix Johnson * 1930</th><td>KT184349</td><td>1763</td><td>46.8</td><td>Ogedengbe et al. (2016)</td></tr><tr><th>DINOFLAGELLATA</th></tr><tr><th>Blastodinium contortum Chatton * 1908</th><td>DQ317537</td><td>1799</td><td>44.8</td><td>Skovgaard et al. (2007)</td></tr><tr><th>Prorocentrum arenarium Faust * 1994</th><td>Y16234</td><td>1788</td><td>46.4</td><td>Grzebyk et al. (1998)</td></tr><tr><th>CILIOPHORA</th></tr><tr><th>LItonotus paracygnus Song* 1994</th><td>EU242509</td><td>1635</td><td>42.0</td><td>Gao et al. (2008)</td></tr><tr><th>Paramecium buestchlii van As et al. * 1998</th><td>KM091234</td><td>1703</td><td>44.2</td><td>Krenek et al. (2015)</td></tr><tr><th>AMORPHEA</th></tr><tr><th>CHOANOZOA</th></tr><tr><th>Salpingoeca infusionum Kent * 1880</th><td>AF100941</td><td>1978</td><td>49.6</td><td>Collins (1998)</td></tr><tr><th>ITS region (OPALINATA)</th><td></td><td></td><td></td><td></td></tr><tr><th>Protoopalina limnocharis</th><td>MN630239</td><td></td><td></td><td>This study</td></tr><tr><th>ITS1</th><td></td><td>231</td><td>2.2</td><td></td></tr><tr><th>5.8S</th><td></td><td>154</td><td>35.7</td><td></td></tr><tr><th>ITS2</th><td></td><td>229</td><td>4.4</td><td></td></tr><tr><th>Protoopalina pingi</th><td>MN630241</td><td></td><td></td><td>This study</td></tr><tr><th>ITS1</th><td></td><td>237</td><td>2.1</td><td></td></tr><tr><th>5.8S</th><td></td><td>154</td><td>36.4</td><td></td></tr><tr><th>ITS2</th><td></td><td>218</td><td>4.6</td><td></td></tr><tr><th>Zelleriella orientalis</th><td>MN630236</td><td></td><td></td><td>This study</td></tr><tr><th>ITS1</th><td></td><td>291</td><td>2.8</td><td></td></tr><tr><th>5.8S</th><td></td><td>167</td><td>26.4</td><td></td></tr><tr><th>ITS2</th><td></td><td>285</td><td>2.5</td><td></td></tr><tr><th>Zelleriella sp.</th><td>MN630237</td><td></td><td></td><td>This study</td></tr><tr><th>ITS1</th><td></td><td>290</td><td>2.8</td><td></td></tr><tr><th>5.8S</th><td></td><td>166</td><td>26.5</td><td></td></tr><tr><th>ITS2</th><td></td><td>279</td><td>2.5</td><td></td></tr><tr><th>Opalina undulata</th><td>MG816209</td><td></td><td></td><td>Li et al. (2018)</td></tr><tr><th>ITS1</th><td></td><td>325</td><td>1.5</td><td></td></tr><tr><th>5.8S</th><td></td><td>167</td><td>24.0</td><td></td></tr><tr><th>ITS2</th><td></td><td>290</td><td>2.4</td><td></td></tr><tr><th>Opalina triangulata</th><td>MK872803</td><td></td><td></td><td>Wang et al. (2019)</td></tr><tr><th>ITS1</th><td></td><td>292</td><td>1.7</td><td></td></tr><tr><th>5.8S</th><td></td><td>167</td><td>24.6</td><td></td></tr><tr><th>ITS2</th><td></td><td>284</td><td>1.8</td><td></td></tr><tr><th>Opalina obtrigonoidea</th><td>MK973090</td><td></td><td></td><td>Zhao et al. (2020)</td></tr><tr><th>ITS1</th><td></td><td>323</td><td>1.6</td><td></td></tr><tr><th>5.8S</th><td></td><td>167</td><td>24.6</td><td></td></tr><tr><th>ITS2</th><td></td><td>287</td><td>1.7</td><td></td></tr><tr><th>Opalina japonica</th><td>MN630238</td><td></td><td></td><td>This study</td></tr><tr><th>ITS1</th><td></td><td>357</td><td>1.7</td><td></td></tr><tr><th>5.8S</th><td></td><td>167</td><td>24.6</td><td></td></tr><tr><th>ITS2</th><td></td><td>358</td><td>2.5</td><td></td></tr><tr><th>Opalina longa</th><td>MN630240</td><td></td><td></td><td>This study</td></tr><tr><th>ITS1</th><td></td><td>283</td><td>1.8</td><td></td></tr><tr><th>5.8S</th><td></td><td>167</td><td>24.6</td><td></td></tr><tr><th>ITS2</th><td></td><td>261</td><td>2.3</td><td></td></tr><tr><th>LSU rDNA (OPALINATA)</th><td></td><td></td><td></td><td></td></tr><tr><th>Protoopalina limnocharis</th><td>MN638759</td><td>3652</td><td>26.7</td><td>This study</td></tr><tr><th>Protoopalina pingi</th><td>MN638761</td><td>3647</td><td>26.9</td><td>This study</td></tr><tr><th>Zelleriella orientalis</th><td>MN638760</td><td>4064</td><td>23.0</td><td>This study</td></tr><tr><th>Zelleriella sp.</th><td>MN638763</td><td>4102</td><td>22.8</td><td>This study</td></tr><tr><th>Opalina undulata</th><td>MN638758</td><td>4108</td><td>22.8</td><td>This study</td></tr><tr><th>Opalina triangulata</th><td>MN638762</td><td>4043</td><td>23.9</td><td>This study</td></tr><tr><th>Opalina obtrigonoidea</th><td>MW504710</td><td>4122</td><td>22.5</td><td>This study</td></tr><tr><th>Opalina japonica</th><td>MN638764</td><td>4120</td><td>22.4</td><td>This study</td></tr><tr><th>Opalina longa</th><td>MN638765</td><td>4096</td><td>23.6</td><td>This study</td></tr></tbody></table>aNishi et al. (2005: 701–702) indicated that ‘the four sequences from Protoopalina sp. were all identical* although they were isolated at different geographical locations.Futhermore* the genus Protoopalina is presently restricted to the host species Rana nigromaculata in Japan.They commonly retain four relatively large nuclei* show similar morphological characters (Table 1)* and are classified as P.japonica (Hara 1934) ’. However* the work by Hara (1934) is on the cell morphology and microtubular structures of Protoopalina axonucleata * and he did not mention any other species in his work.According to the statements by Nishi et al. (2005) * their isolates corresponded to the same species studied by Hara (1934) and should be identified as P.axonucleata. The name Protoopalina japonica is a nomen nudum because this species has never been described.

ShareScore

32/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship: 8
Harmonization: 8
Access: 8
Reuse readiness: 8
Engagement: 0

Topics

Biodiversity Taxonomy