Active fluctuations of axoneme oscillations scale with number of dynein motors

<p>&nbsp;</p> <p>The dataset published here was used to measure the<strong> phase fluctuations</strong> <strong>(frequency jitter) </strong>of isolated and <strong>reactivated axonemes from <em>Chlamydomonas reinhardtii&nbsp;</em></strong>as a function of the dynein motor density in these axonemes. <br>The corresponding article is published in PNAS: https://doi.org/10.1073/pnas.2406244121</p> <p>Axonemes were isolated from wt(cc-125) and oda1(cc-2228) cells.</p> <p>Reactivation was performed:<br>(1) after dynein extraction with different amounts of salt (KCL) or<br>(2) in the presence of different concentrations of ATP .</p> <p>For all experimental conditions we provide the following data files:<br><br></p> <p><strong>Movies of reactivated axonemes:</strong></p> <p>Reactivated axonemes were imaged with phase-contrast microscopy.&nbsp;Recorded movies are organized in folders (.zip) which are labeled according to the respective reactivation condition (KCL and ATP concentration as well as the <em>Chlamydomonas</em> strain, from which the axonemes were purified, e.g. <strong>50mM_KCl_750uM_ATP_ODA_Extracted.zip</strong>).</p> <p>Those folders contain (1) movie files (multilayer .tif) of single axonemes and (.txt) files with a corresponding number-label (with microscope, camera settings and experimental condition (<em>Chlamydomonas</em> strain, [ATP], [KCL]).</p> <p>Using the number-label, the corresponding data file can be identified. &nbsp;<br><br></p> <p><strong>Data files (for the corresponding movies):</strong></p> <p>The experimental data is organized in MATLAB (.mat) files. Those files contain shape and waveform information (see below) for the respective reactivation condition (axoneme type, [ATP], [KCL] detailed below the file name).</p> <p><strong>WT_KCL_master.mat</strong> - WT axonemes, KCL extracted and reactivated with 750uM ATP</p> <p><strong>0mM_KCl_750uM_ATP_WT_Extracted<br>50mM_KCl_750uM_ATP_WT_Extracted<br>100mM_KCl_750uM_ATP_WT_Extracted<br>200mM_KCl_750uM_ATP_WT_Extracted<br>300mM_KCl_750uM_ATP_WT_Extracted<br>400mM_KCl_750uM_ATP_WT_Extracted<br></strong></p> <p><strong>WT_ATP_master.mat&nbsp;</strong> - WT axonemes reactivated with different ATP concentraions</p> <p><strong>0mM_KCl_50uM_ATP_WT<br>0mM_KCl_100uM_ATP_WT<br>0mM_KCl_370uM_ATP_WT<br>0mM_KCl_500uM_ATP_WT<br>0mM_KCl_750uM_ATP_WT<br></strong></p> <p><strong>ODA_KCL_master.mat </strong>- ODA axonemes, KCL extracted and reactivated with 750uM ATP</p> <p><strong>0mM_KCl_750uM_ATP_ODA_Extracted<br>50mM_KCl_750uM_ATP_ODA_Extracted<br>100mM_KCl_750uM_ATP_ODA_Extracted<br>200mM_KCl_750uM_ATP_ODA_Extracted<br>300mM_KCl_750uM_ATP_ODA_Extracted<br></strong></p> <p><strong>ODA_ATP_master.mat</strong> - ODA axonemes reactivated with different ATP concentraions</p> <p><strong>0mM_KCl_70uM_ATP_ODA<br>0mM_KCl_100uM_ATP_ODA<br>0mM_KCl_370uM_ATP_ODA<br>0mM_KCl_500uM_ATP_ODA<br>0mM_KCl_750uM_ATP_ODA</strong></p> <p>Each file includes a data-cell &lsquo;Master&rsquo; with a columns for the different experimental conditions (e.g. concentrations of ATP or KCL). Data-cells contain one structure for each axoneme.&nbsp;</p> <p>&nbsp;</p> <p>These structures have the following fields:</p> <p>nframe&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; number of frames of the dataset</p> <p>phi_in_rad&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; phase angle (beat-cycle-phase) (rad)</p> <p>tangent_angle_psi_in_rad&hellip;&nbsp;&nbsp; the tangent angle for 24 positions along arc-length (rad)</p> <p>xy_in_micron&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; the x,y positions (&micro;m)</p> <p>dt_in_second&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; time between adjacent frames (second)</p> <p>ds_in_micron&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; spacing between adjacent arc-length positions (&micro;m)</p> <p>f0_in_Hz&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; beat frequency (Hz)</p> <p>A&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; beat amplitude (arc-length average) (rad)</p> <p>Q&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; quality factor</p> <p>sexp&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; string with experiment label</p> <p>KCL_in_mM&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; KCL concentration (mM) used for dynein extraction</p> <p>ATP_in_uM&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; ATP concentration (mM) used for reactivation</p> <p>File&hellip;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; filename, includes a number-label that corresponds to the imaging data provided</p>