A single high‑zinc activation enhancer can control two genes oriented head-to-head in C. elegans

<p>Supplementary Data for paper article, Divergent Enhancer Function of the High-Zinc Activation Element in&nbsp;<em>C. elegans</em>". Supplementary File 1 contains all qPCR data processing spreadsheet (eg. Organized Data Cd, hizr-1 am286, Zn, etc), GraphPad Prism sheets, and log2 average calculation sheets (for superplot). Corresponding folders to figures: &ldquo;hizr-1 am286&rdquo; &ndash; Fig 1, &ldquo;Zn&rdquo; &ndash; Fig 2 &amp; 3, Sup Fig S1, S3, &amp; S5, &ldquo;Cd&rdquo; &ndash; Sup Fig S2, S4, &amp; S5, &ldquo;mtl-2&rdquo;, &ldquo;srh-308&rdquo; &ndash; Fig 4. Supplementary File 2 contains sequencing files for HZA^mT and HZA^cF loci. &ldquo;cdr-1_sequencing.seq&rdquo; and &ldquo;mtl-2_sequencing.seq&rdquo; are Sanger sequencing results for the knockout strains. &ldquo;mtl-2 gk125&rdquo; folder contains Sanger sequencing results for gk125 allele verification. Supplementary File 3 contains sequences of <em>mtl-2</em> homologs from <em>C. breneri</em>, <em>C. briggsae</em>, and <em>C. remanei</em> used for alignment in Fig 2.</p>