Fig. 4 in Nutritional restriction triggers callose accumulation on the sieve plates of the funiculus of developing bean seeds

Fig. 4. Distribution and metabolic use of sucrose [U-14C] in the bean fruits.Total incorporation of sucrose [U-14C] by fruits (A) and the proportion allocated in seeds (B) or in individual seeds (C).A full bar corresponds to fruits at 23 DAA that developed under normal conditions, and gray bars indicate fruits of the same age that were removed from the plant three days before the label experiment (Removed). The metabolic use of sucrose in cotyledons was also studied (D). In this case, the full bar represents the label that was soluble in 80% ethanol; the gray bar indicates the amount of radioactivity recovered from starch, and the open bar represents the label recovered from proteins.For fruits that had been removed from the plant,only seeds that were able to continue their development were considered. Bars correspond to the average of analysis of three fruits from different plants ± SD. NS indicate no significant difference, *, ** and *** stand for significant differences at P <.05, 0.01, and 0.001, respectively; as indicated by t test for A and B, Mann–Whitney test for C, and ANOVA for D.