Fig. 6 in Gene identification using RNA-seq in two sweetpotato genotypes and the use of mining to analyze carotenoid biosynthesis

Fig. 6. qRT-PCR analysis of the selected genes associated with carotenoid and terpenoid backbone biosynthesis pathways. Roots, stems and leaves were collected, and the total RNAs was extracted, respectively, pooled and used in qRT-PCR analysis of the genes FDPS, PSY, HMGCR, HMGCS1, CRTISO and HDR. Relative expression levels were calculated using the 2−ΔΔCt method, with actin as an internal control.