Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature

<p>This dataset contains pre-processed untargeted GC-MS metabolomics and direct shotgun proteomics from a microcosm woodblock experiment described in the paper "Rawlings et al (2021) Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature. Ecology."</p> <p>The experiment investigates the effect of diurnal cycling of temperature on the metabolism of two wood decay species, <em>Mucidula mucida</em> and <em>Exidia glandulosa</em>. We colonised beech woodblocks with the two species (separate microcosms) and exposed them to either a diurnally cycling (mean 15 ± 10°C) or constant (15°C) temperature, in a fully factorial design. After 8 weeks we extracted the full complement of metabolites and proteins in the wood to examine the extent to which the abundances of metabolic products were altered.</p> <p>The main results of the experiment were that products linked to lignin breakdown, the citric acid cycle, pentose phosphate pathway, carbohydrate metabolism, fatty acid metabolism and protein biosynthesis and turnover were under-enriched in fluctuating, compared to stable temperatures, in the generalist <i>M. mucida</i>. Conversely <i>E. glandulosa</i> showed little differential response to the experimental treatments which may be linked with its preference for habitats exhibiting abiotic stress. </p>