FIGURE 4 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)

FIGURE 4. Sexual stage of Shiraia bambusicola (epitype). (Figs A–D, F–M, O–P light microscopy and Figs E, N SEM). A. Ascostroma with yellow ascospore mass. B. Cross section of ascostroma. Note the sexual locules lining the periphery (a) and the asexual locules near the centre (b). The outer part is pink, while the inner part is white to light pink. C. Cross section of locules (fluorescent microscopy stained in Hoechst 33258). D. Vertical section of ascostroma. E. Tissue of locule wall. Note the cells of textura angularis at the inside fusing with cells of textura intricata at the outside. F. Pseudoparaphyses (cotton blue reagent staining). G. Asci and pseudoparaphyses. H–K. Asci. L–M. Apices of asci showing ocular chamber. N–P. Ascospores (P stained in lactic acid phenol Medan). Scale bars: A = 1 cm; B = 1.5 cm; C–D = 100 µm; E = 10 µm; F = 20 µm; G–K = 50 µm; L–M = 20 µm; N–P = 10 µm.