FIGURE 6 in MIDTAL (Microarrays for the Detection of Toxic Algae)

FIGURE 6: Scan image of a portion of one of the grids (left panel, see also the lower panel in Fig. 2) in the first generation MIDTAL microarray after hybridization with Cy5-labelled RNA extracted from a field sample collected in the Skagerrak coast (Gullmarnfjord, Sweden) in the beginning of August 2009. An analysis of the hybridisation was performed with the PhyloChip program, and a portion of this analysis is presented in the histogram (right panel). Each cluster of four dots in the left panel represents the same probe spotted four times as shown in Fig. 2 lower panel. Fluorescence values in the right panel are arranged from lowest to highest signal strength. The red line across the intensity values marks the threshold over which a positive signal is recorded. Stars indicate a significant signal for species level probe of two toxic species Karenia brevis (Davis) G. Hansen et Moestrup in Daugbjerg et al. (2000: 308) (KB5) and Pseudo-nitzschia multistriata (Takano) Takano (1995: 73) (mD3) present in the sample, seen both with the microarray and traditional counts. Other high signals include the probes for the positive control Dunaliella and other higher group level probes, such as EUK1209, a probe for all eukaryotes and HETERO01, a probe for all heterokonts. Error bars represent duplicate hybridisation of the same field sample (two grids hybridised at the same time and signals averaged).