Figure 3 in Five invaginations and shedding of the larval epidermis during development of the hoplonemertean Pantinonemertes californiensis (Nemertea: Hoplonemertea)

Figure 3. Confocal micrographs of phalloidin-labelled early (A, B) and late (C, D) invagination stage larvae of Pantinonemertes californiensis. (A, B) A 28.5-hour-old prehatched embryo (12–13◦C); (C, D) a 46-hour-old (12–13◦C) larva; apical pole marked by the apical plate (ap) is up or upper right. (A) Confocal Z-projection (ventral view) showing stomodeum (st), proboscis rudiment (pb), paired anterior (ai) and posterior (pi) invaginations; (B) a 30-µm thick sub-stack of frontal sections showing the posterior cirrus (pc), paired anterior and posterior invaginations, proboscis and midgut lumen (arrowhead); (C) confocal Z-projection (ventral view), showing bifurcated anterior and posterior invaginations and the stomodeum (st); (D) a 29-µm thick sub-stack of frontal sections showing the five invaginations and midgut lumen (arrowhead).