FIGURE 3. The strict consensus tree from parsimony analysis for the combined 16S and RAG1-AmpF1 in Maluti Mystery: A systematic review of Amietia vertebralis (Hewitt, 1927) and Strongylopus hymenopus (Boulenger, 1920) (Anura: Pyxicephalidae)

FIGURE 3. The strict consensus tree from parsimony analysis for the combined 16S and RAG1-AmpF1 dataset with Pyxicephalus adspersus as the outgroup. Bootstrap values are shown at the major nodes. Branch lengths are proportional to the number of unambiguous changes in the original sequence data. Abbreviations of localities for sequenced samples are as follows: M = Mohlaka, B = Bafali, D = Senqu, Q = Qabane, S = Tsatsana, T = Tugela, V = Vemvane, J = Sani, * = Genbank sequence. Note that the two Amietia angolensis sequences from Genbank emerge from the tree as divergent lineages. This may indicate cryptic diversity or simply misidentification of these phenotypically diverse and difficult to identify frogs.