FIGURE 2 in Non-invasive ancient DNA protocol for fluid-preserved specimens and phylogenetic systematics of the genus Orestias (Teleostei: Cyprinodontidae)

FIGURE 2. Gel electrophoresis displaying extracted DNA (a) and amplified PCR fragments (b) in two historical samples of O. mulleri (MNHN 1981-1428#4 and #13). In a, columns 1–2: extracted DNA for specimen #4 (aliquots a and b); columns 3–4: extracted DNA for specimen #13 (aliquot a and b); column 5: negative DNA extraction control; M: molecular weight marker (100 bp ladder). In b, PCR amplification products for control region and rhodopsin are left and right to the molecular weight marker, respectively. Columns 1–2: specimen #4 (aliquots a and b); columns 3–4: specimen #13 (aliquots a and b); column 5: negative PCR control; column 6: positive PCR control. M: molecular weight marker (100 bp ladder).