Fig. 23 in Hybridization Among Western Whiptail Lizards (Cnemidophorus Tigris) In Southwestern New Mexico: Population Genetics, Morphology, And Ecology In Three Contact Zones

Fig. 23. Dot­blot illustrating specificity of the allele­specific oligonucleotide probes (ASOs) DNA samples from 24 lizards from 12 different sites were applied in rows A and C of a strip of nitrocellulose paper. After heat denaturation, the blot was hybridized successively with ASO­PUN (Tucson) (Top), ASO­Mar (Middle), and ASO­ PUN (Bottom). Note that C. t. punctilinealis samples from sites 49 and 56, west of the contact region, hybridized only to ASO­PUN (Tucson (positions 1–3 of row A), in contrast to samples of punctilinealis from the contact region, which hybridized only with ASO­PUN (positions 5 and 7 of row C). DNA of C. t. marmoratus from all sites paired with ASO­MAR (positions 4–12 of row A, and positions 1–4, 6, and 8–12 of row C)