RNA sequencing of PET127 mutants overexpressed using the galactose inducible promoter

<p>In Saccharomyces cerevisiae, <em>PET127</em> is a mitochondrial RNA exonuclease known to interact with the mitochondrial RNA polymerase <em>RPO41</em>. The nature of the interaction between <em>PET127</em> and <em>RPO41</em> is unclear. Here, we overexpressed different <em>PET127</em> mutants for five hours utilizing the galactose inducible promoter on high-copy plasmids, collected samples, and performed RNA sequencing to understand the mitochondrial RNA landscape in each condition. The mutants tested were <em>PET127</em> lacking the nuclease activity (<em>pet127-nd</em>) and two truncated forms of <i>PET127</i>: either lacking the <em>RPO41</em> binding region (<i>pet127∆(48-215</i>)), or only containing the <i>RPO41</i> binding region (<em>pet127(1-215)</em>).</p>