Potential Cell Lysis Mechanisms for B. subtilis

<p><strong>Description:</strong></p> <p>A list of potential cell lysis mechanisms for Bacillus subtilis, cured and organized as part of the partnership between iGEM UANL and UNILA teams in the design of a Kill Switch for B. subtilis. For more information on both designs, check out the projects wikis:</p> <p>iGEM UNILA LatAm 2021:&nbsp;https://2021.igem.org/Team:UNILA_LatAm</p> <p>iGEM FCB-UANL 2021:&nbsp;https://2021.igem.org/Team:FCB-UANL</p> <p><strong>Columns:</strong></p> <ul> <li><strong>CRISPR:</strong>&nbsp;CRISPR systems developed for B. subtilis <ul> <li><strong>CRISPR Variant:&nbsp;</strong>Cas variant used in the system</li> <li><strong>Editing/Disruption Efficiency:&nbsp;</strong>System efficiency evaluated for multiple or single editions</li> <li><strong>Mechanisms:&nbsp;</strong>operating mechanism</li> <li><strong>Application:&nbsp;</strong>Main objective for the developed system</li> <li><strong>B. subtilis considerations:&nbsp;</strong>Particularities regarding the development of the system in B. subtilis</li> <li><strong>Year:&nbsp;</strong>year of publication</li> </ul> </li> <li><strong>TA Systems:</strong>&nbsp;Toxin-Antitoxin systems developed for B. subtilis&nbsp; <ul> <li><strong>Toxin-Antitoxin:&nbsp;</strong>Which antitoxins and toxins involved in the system&nbsp;&nbsp;</li> <li><strong>Type: </strong>TA System Type</li> <li><strong>Mechanism:&nbsp;</strong>operating mechanism</li> <li><strong>Application:&nbsp;</strong>Main objective for the developed system</li> </ul> </li> <li><strong>Sporulation: </strong>Control of sporulation events in B. subtilis&nbsp; <ul> <li><strong>Spores Control</strong></li> <li><strong>Type</strong></li> <li><strong>Mechanism:&nbsp;</strong>operating mechanism</li> <li><strong>Application:&nbsp;</strong>Main objective for the developed system</li> </ul> </li> <li>&nbsp;<strong>iGEM Applications:</strong> Kill Switch designs for B. subtilis since 2014. <ul> <li><strong>Kill Switch System:&nbsp;</strong>Design description of Kill switch systems</li> <li><strong>Type: </strong>System components</li> <li><strong>Mechanism:&nbsp;</strong>operating mechanism</li> <li><strong>Application:&nbsp;</strong>Main objective for the developed system</li> <li><strong>Test:&nbsp;</strong>Type of assessment performed by the team</li> <li><strong>Parts Registry: </strong>Parts submitted to the Registry of Standard Biological Parts.</li> </ul> </li> </ul> <p><strong>Remote Access:</strong>&nbsp;<a href="https://docs.google.com/spreadsheets/d/1PM7NS5JbfVLgjsa6s7L8yqBj_G1A5mSnKCPaeGmkIwg/edit#gid=0">Potential Cell Lysis Mechanisms for B. subtilis</a></p> <p>&nbsp;</p> <p>&nbsp;</p> <p>&nbsp;</p>