Figure 1 from: Nilsson R, Tedersoo L, Abarenkov K, Ryberg M, Kristiansson E, Hartmann M, Schoch C, Nylander J, Bergsten J, Porter T, Jumpponen A, Vaishampayan P, Ovaskainen O, Hallenberg N, Bengtsson-Palme J, Eriksson K, Larsson K, Larsson E, Kõljalg U (2012) Five simple guidelines for establishing basic authenticity and reliability of newly generated fungal ITS sequences. MycoKeys 4: 37-63. https://doi.org/10.3897/mycokeys.4.3606

Figure 1 - An ITS alignment featuring five random species each of the fungal phyla Ascomycota, Basidiomycota, Glomeromycota, Chytridiomycota, and Zygomycota s.l. The left half of the screen represents the ITS1 and the right half the 5.8S. Whereas the ITS1 alignment appears more or less chaotic, the 5.8S stands out as a very conserved element throughout these five phyla. The 5.8S starts at position 803 (indicated by the black cursor in the uppermost sequence). Seaview (Gouy et al. 2010) was used to display the alignment.