Mechanisms Governing Target Search and Binding Dynamics of Hypoxia-Inducible Factors

<p>Raw fast SPT data for Hypoxia-Inducible Factors:</p> <p><strong>HIF2a_HIF1b_KIN_Clones.zip </strong>corresponds to Figure 2A-C and Figure 2-Figure supplement 2A-B, contains fast SPT data for Halo-HIF-2&alpha; KIN clone A31 and clone B50, Halo-HIF-1&beta; KIN clone A21 and clone B89.</p> <p><strong>HIF1b_with_HIF2a_overexpression.zip </strong>corresponds to Figure 2D-E and Figure 2-Figure supplement 2C, contains fast SPT data for: Halo-HIF-1&beta; KIN clone A21, Halo-HIF-1&beta; KIN clone A21 with HIF-2&alpha; stably over-expressed, and Halo-HIF-1&beta; KIN clone A21 with a dimerization mutant form of HIF-2&alpha; (R171A/V192D) stably over-expressed.</p> <p><strong>Belzutifan_dosage.zip </strong>corresponds to Figure 3-Figure supplement 1, contains fast SPT data for: Halo-HIF-2&alpha; KIN clone A31, treated with 0.0002 uM, &nbsp;0.002 uM, 0.005 uM, 0.01 uM, 0.02 uM, and 0.2 uM of Belzutifan.</p> <p><strong>HIF1b_with_HIFa_chimera_overexpression_and_Belzutifan.zip </strong>corresponds to Figure 3 and Figure 3-Figure supplement 2, contains fast SPT data for: Parental Halo-HIF-1&beta; KIN clone A21 cells and cells stably over-expressing either a certain form of HIF-&alpha; (WT or domain swap), treated with either Belzutifan or DMSO.&nbsp;</p> <p><strong>HIFa_domain_swap.zip </strong>corresponds to Figure 4 and Figure 4-Figure supplement 2, contains fast SPT data for: Halo-HIF-2&alpha; KIN clone A31, WT 786-O cells stably express V5-Halo-HIF-2&alpha; with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-2&alpha;/1&alpha; with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-1&alpha; with an L30 promoter, and WT 786-O cells stably express V5-Halo-HIF-1&alpha;/2&alpha; with an L30 promoter. &nbsp;</p> <p><strong>DBD_mutant.zip </strong>corresponds to Figure 5A-C, contains fast SPT data for: WT 786-O cells stably express V5-Halo-HIF-2&alpha; with an L30 promoter, WT 786-O cells stably express a DBD mutant form of V5-Halo-HIF-2&alpha; (R27E) with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-1&alpha; with an L30 promoter, and WT 786-O cells stably express a DBD mutant form of V5-Halo-HIF-1&alpha; (R30E) with an L30 promoter.</p> <p><strong>Dimerization_mutant.zip </strong>corresponds to Figure 5D-F, contains fast SPT data for: WT 786-O cells stably express V5-Halo-HIF-2&alpha; with an L30 promoter, WT 786-O cells stably express a dimerization mutant form of V5-Halo-HIF-2&alpha; (R171A/V192D) with an L30 promoter, WT 786-O cells stably express V5-Halo-HIF-1&alpha; with an L30 promoter, and WT 786-O cells stably express a dimerization mutant form of V5-Halo-HIF-1&alpha; (R170A/V191D) with an L30 promoter.<br> &nbsp;</p>